Applications

Flow Cytometry

Pavilion Integration’s WhisperIT® ultra low-noise, low-coherence, mode-hop free lasers and Comet® solid-state detectors enable detection of small particle extracellular vesicle detection as well as marine cyanobacteria throughout the oceans, 1/10th the size of a human cell.

Flow cytometry is a technology that provides rapid multi-parametric analysis of single cells in solution. PIC WhisperIT® lasers produce both scattered and fluorescent light signals that are read by detectors such as photodiodes, photomultiplier tubes or PIC Comet® solid-state detectors. These signals are converted into electronic signals that are analyzed by a computer and written to a standardized format (.fcs) data file. Cell populations can be analyzed and/or purified based on their fluorescent or light scattering characteristics.

A variety of fluorescent reagents are utilized in flow cytometry. These include, fluorescently conjugated antibodies, DNA binding dyes, viability dyes, ion indicator dyes and fluorescent expression proteins.

Flow cytometry is a powerful tool that has applications in immunology, molecular biology, bacteriology, virology, cancer biology and infectious disease monitoring. It has seen dramatic advances over the last 30 years, allowing unprecedented detail in studies of the immune system and other areas of cell biology.


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